Application

Research Sub CategoryNeurochemistry & NeurotrophinsGrowth Cones & Axon Guidance

Western blot: 1-10 µg/mL using ECL. The calculated molecular weight of Nogo-A is approximately 135 kDa. However a molecular weight of approximately 180 kDa has been reported for full length Nogo-A (Chen et al. 2000; GrandPre et al. 2000; Goldberg & Barres 2000; Prinijha et al. 2000; Tessier-Lavigne & Goodman 2000; Nagase et al. 1998).

ELISA: 1:10,000-1:100,000 using 50-100 ng control peptide (Catalog Number AG348) per well.

Optimal working dilutions must be determined by the end user.

Research CategoryNeuroscience

Anti-Neurite Outgrowth Inhibitor A Antibody is an antibody against Neurite Outgrowth Inhibitor A for use in ELISA & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

An 18 amino acid peptide sequence in the middle region of the putative extracellular domain of rat Nogo-A (Chen et al. 2000; GrandPre et al. 2000; Goldberg & Barres 2000; Prinijha et al. 2000; Tessier-Lavigne & Goodman 2000; Nagase et al. 1998).

The immunogen peptide is highly conserved in mouse (94%) and human (83%) Nogo-A.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB5888

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Liquid in PBS containing 0.1% BSA.

Specificity

Recognizes rat Neurite Outgrowth Inhibitor Protein A (Nogo-A). The immunogen shows no significant sequence homology with the alternatively spliced shorter forms Nogo-B or Nogo-C.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months afet date of receipt. Avoid repeated freeze/thaw cycles.